An automated p-nitrophenylphosphate serum alkaline phosphatase procedure for the AutoAnalyzer.
نویسندگان
چکیده
A new and simplified procedure for the automated determination of serum alkaline phosphatase uses the AutoAnalyzer. The substrate p-nitrophenylphosphate in 2amino-2-methyl-l-propanol offers the prime advantage of providing directly its own chromogen, p-nitrophenol, following enzyme action. The procedure also permits use of small sample volumes, provides linear reaction rates, and has a simple manifold design. Correlations are presented between the manual procedure and the automated system.
منابع مشابه
An automated p-nitrophenylphosphate serum alkaline phosphatase procedure for the "Robot Chemist".
A new, precise, and accurate procedure is described for the automated determination of serum alkaline phosphatase on the Robot Chemist. The substrate, p-nitrophenylphosphate in 2-amino-2-methyl-1-propanol, offers the prime advantage of providing directly its own chromogen, p-nitrophenol, following enzyme action. Other advantages are the small sample volumes required and linear reaction rates. C...
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ورودعنوان ژورنال:
- Clinical chemistry
دوره 11 9 شماره
صفحات -
تاریخ انتشار 1965